The combination of high throughput sequencing with crosslink immunoprecipitation makes it possible to identify direct in vivo interaction partners, and binding motifs of RBPs and their target mRNA. This knowledge will prove vital in the elucidation of the critical role RNA-binding proteins play in the function, biogenesis and cellular localization of RNAs.
In October 2011 I joined the COAT project as a PhD student in the group of Jan Christiansen. I work with adapting the UV-crosslinking and immunoprecipitation (CLIP) technology to a Drosophila melanogaster cell line, with the purpose of identifying the exact mRNA targets of a cytoplasmatic RNA binding protein involved in the development and maintenance of the embryonic nervous system.
I have a Masters degree in Molecular Biomedicine from the University of Copenhagen, and have previously worked in the Section for Functional Genomics where I investigated the regulation of non-coding RNA transcription in relation to the heterochromatic regions in yeast.